Total Yeast & Mold
Total Yeast & Mold Count (TYMC)
The Total Yeast and Mold Count (TYMC) is a microbiological test used to estimate the total number of viable yeast and mold cells present in a sample. TYMC is an important indicator of product quality and safety, particularly for items susceptible to fungal growth such as foods, beverages, dietary supplements, cosmetics, plant-based products, and water-sensitive formulations.
In a TYMC analysis, the sample is diluted in a sterile diluent and plated onto an agar medium specifically formulated to support the growth of yeast and mold. After incubation, the resulting colony-forming units (CFUs) are counted and reported as CFU per gram or per milliliter of the original sample.
While TYMC and TAPC are both measures of microbial load, TYMC is specialized:
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TYMC targets only yeast and mold, organisms that thrive in low-moisture and nutrient-variable environments.
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TAPC measures total aerobic microorganisms, including bacteria, yeast, and mold.
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Different media are used: TAPC commonly uses Tryptic Soy Agar (TSA), whereas TYMC uses selective media such as Sabouraud Dextrose Agar (SDA), Potato Dextrose Agar (PDA), or other fungal-supportive formulations.
Although TYMC is an effective overall indicator of fungal contamination, it does not identify the species present. When specific yeast or mold organisms are of concern—such as Candida, Aspergillus, Penicillium, or emerging spoilage organisms—additional identification testing may be required.
Common compendial methods for TYMC include:
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USP <61>
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USP <2021>
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European Pharmacopoeia (EP) 2.6.12
At Unicorn Analytical, we provide precise and reliable Total Yeast & Mold Count testing using compendial and customized methodologies. Our microbiology team works closely with clients to select the most appropriate method for their product type and regulatory requirements.
Contact Unicorn Analytical today to discuss how we can support your TYMC testing needs!